| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2331 | Thermobifida fusca | ACGGCTTGGTCAACGTCTT | GAATGGCCTTGTGCACGTG | 59.85 | 60.08 | 231 | 47 |
100.00%
|
100.00%
|
| Primer 2332 | Thermobifida fusca | GCGATGGATACCACGGCTT | AATGGCCTTGTGCACGTG | 60.23 | 58.96 | 242 | 47 |
100.00%
|
100.00%
|
| Primer 2333 | Thermobifida fusca | TCTGCCTCAGCTTGTACGC | GGGTCGTCTTTGATGCGGA | 60.08 | 60.08 | 199 | 47 |
100.00%
|
100.00%
|
| Primer 2334 | Thermobifida fusca | TCTCGCGTTGACGGAGAAG | TTCAGTTCCACCGTCTCGC | 59.79 | 60.01 | 246 | 47 |
100.00%
|
100.00%
|
| Primer 2335 | Thermobifida fusca | AACGACACCAGGCACAGT | GGTCGTCTTTGGCGTCCTT | 59.09 | 60.30 | 262 | 47 |
100.00%
|
100.00%
|
| Primer 2336 | Thermobifida fusca | CGCCACATCGTCTACCTGT | TGCGGTTGACGTGCTCTT | 59.49 | 59.89 | 280 | 47 |
100.00%
|
100.00%
|
| Primer 2337 | Thermobifida fusca | GCGATCCTCAACGAGCAGA | TGGCGAGGCTCTGGTAGAT | 59.86 | 60.08 | 187 | 47 |
100.00%
|
100.00%
|
| Primer 2338 | Thermobifida fusca | TTTCGACCTTGCCGTGGT | GCCTTTCCTCAGTCGGCTT | 59.49 | 60.00 | 155 | 47 |
100.00%
|
100.00%
|
| Primer 2339 | Thermobifida fusca | AGAACAGTTCCACCACCGC | AGCTTCTCGCGTCGTGAA | 60.23 | 59.36 | 293 | 47 |
100.00%
|
100.00%
|
| Primer 2340 | Thermobifida fusca | ACCTCCAGGATTTGACCGC | AAACCCACACGAGTCCCAC | 59.70 | 59.85 | 195 | 47 |
100.00%
|
100.00%
|